Just a reminder about the fluoromter
1) choice of excitation/emission
If using Hoechst dye in the test protocol set the Excitation at 350nm and the Emission at 460nm
For PicoGreen use 485 Ex. and 545 Em.
2) Dynamic range of the dye
Ensure that the DNA you are wanting to quantitate is within the dynamic range of the dye/concentration you are using and that the standard curve is linear within that range.