sent on behalf of Terry, following on last Fridays’s TAB group meeting.
Attached are the proofs for Kenta et al’s multiplex SNP-Scale paper. (A few typos are not corrected.)
The paper includes a number of techniques (not all necessarily original) that may be of wider interest beyond the SNP studies that it focuses on, for example:
The use of 2-ul PCR volumes – it should be a useful source reference for this.UFOs – Universal Fluorescent Oligonucleotides – these might be worth considering as an economical way of generating primers to use in the otherwise expensive ABI 5-dye set, or as a way of easily altering the dye attached to each primer when creating new multiplexes (no need to re-order primers).
PCR trouble shooting, including:
Use of Touchdown PCR program