Sequencing- use ‘real controls’ if possible

Hi folks- just a note about DNA sequencing- if possible use one ‘real control’ (i.e. samples that you know have already worked for a particular primer as well as a standard pGEM). You could ask a colleaugue also for a sample if you do not have one?

The working or otherwise of this sample will give more info. to guide you to the source of any problem (see my email regarding failing reverse sequences).

cheers

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