Plate based Ammonium Acetate DNA extractions- CARE!!

…as mentioned in a recent meeting please be aware of changes to the protocol for carrying out this technique- note that extreme care is needed to prevent leakage from the strips of eight tubes (importantly take care when inverting the tubes when hot and absolutely DO NOT rotate in the oven). 37 degrees C. is better- and you may also need to ‘bleed’ them by opening the lids during heating to equalise the pressure). Anyway- you get the idea…

The technique is not perfect- but with a bit of care as above does work- mke sure you get an experienced buddy to help you 1st.  if you want to adapt it or source better tubes then please do so.

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