1) NED markers?- I am getting a ‘feeling’ that they are invariably weaker/failing more than we should expect? Is that correct- anyone got any empirical evidence? To counteract this you may need to increase the amount of NED primers in your mastermixes (test your ‘plexes!).
2) Occasionally we do see ‘blibs’ of bad data on the 3730 – we have had a couple of plates recently. Do keep your eye on this and try to find the reason why (a look at the raw data picture in Run History can be useful- and of course duplicates/standard samples).
3) Multichannel pipettes. Sam and I have tested/fixed the multichannel pipettes. All should be good. Let us know if not and we will have a closer look.