qPCR machine fine-failed melt curve info

I have spoken to technical support about a few recent runs that had failed melt curves, and they told me all was fine with the instrument and the software-so everything is fine, continue using as normal. :-)
Just so noone makes the same mistake as me, where I went wrong … I was doing a relative standard curve using the advanced setup to use different temp zones, and had assigned the target, selected ‘standard’, and imput the quantity, for each well, but each well must also have a sample assigned to it too (all wells can contain the same sample) in Setup>Plate setup>assign targets and samples, in order for the analysis to complete correctly. (you can go back and do this after the run is complete if neccessary and will be able to retrieve your data). Hope that’s helpful/not too confusing?!
Laura

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